Deciphering the impact of contaminating microbiota in DNA extraction reagents on metagenomic next-generation sequencing workflows

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Deciphering the impact of contaminating microbiota in DNA extraction reagents on metagenomic next-generation sequencing workflows

mNGS 工作流程中汙染來源的動態特性。研究強調，試劑背景並非恆定不變，而是隨不同廠牌及製造批次而波動；因此，實驗室必須針對每一批次實施監控。此外，透過比較健康個體血液樣本與對照組，研究確認血液中的微生物讀數主要源自外源性汙染，而非來自人體內源性微生物。最終建議以「萃取空白」取代健康血液，作為常規檢測的標準陰性對照，以優化病原體鑑定的準確性。

全文連結 : https://pubmed.ncbi.nlm.nih.gov/40833093/

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Deciphering the impact of contaminating microbiota in DNA extraction reagents on metagenomic next-generation sequencing workflows

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